(a) Steward Linn (b) Stanley Cohen (c) Paul Berg (d) Kary Mullis. "Polymerase Chain Reaction" quiz questions and answers PDF: Steps involved during PCR includes, with answers to enroll in online classes. Researchers are obtaining large number of specific pieces of DNA for experimental and diagnostic purposes. It includes MCQ questions on computer memory and types of memory, parts of central processing unit, printing services, computer networking and characteristics of peer to peer networking included in computer fundamentals. When the curves reach a plateau, amplification stops due to the consumption of all primer or TaqMan probe molecules (MCQ 17: D); at this point, the copy number of PCR products is identical in the two samples (MCQ 15: C). High-Resolution Melting (HRM) analysis is a relatively new, post-PCR analysis method used to identify variations in nucleic acid sequences. It remains active at high temperature. Each MCQ type question has four choices out of which only one choice is the correct answer. CORRECT ANSWERS EXPLANATIONS Taq polymerase is a heat-resistant DNA polymerase widely used in PCR protocols. Section – A contains a total of 30 Multiple Choice Questions (MCQ). Start studying MCQ on PCR (Polymerase Chain Reaction. PCR reaction efficiency is dependent on the master mix performance, the specificity of the primers, the primer annealing temperature, and the sample quality. Contents• What is PCR?• History of PCR• Components of PCR• Principles of PCR• Basic Requirements• Instrumentation• PCR Programme• Advantages of PCR• Applications of PCR 3. This quiz and attached worksheet will help gauge your understanding of the Sanger method of DNA sequencing. Students can solve NCERT Class 12 Biology Biotechnology: Principles and Processes MCQs Pdf with Answers to know their preparation … PCR 56. About This Quiz & Worksheet. MCQ on Different types of PCR. In DNA finger printing, the DNA from the gel is transferred to _____ for hybridization 1. nitrocellulose membrane 2. agarose 3. autoradiogram 4. … Teaching. PCR is used for making sequencing templates 10. In general, PCR efficiency above 90% is acceptable. In RACE, 5’ or 3’ ends of the mRNA are copied into cDNA and are based on one primer specific to an internal site of the mRNA. Multiple choice questions with Answers. In AP PCR, a short arbitrary primer is used to amplify multiple fragments of DNA from the whole genome isolate of an organism to get a DNA fingerprint. We have provided Biotechnology and its Applications Class 12 Biology MCQs Questions with Answers to help students understand the concept very well. Southern blotting is a technique used in genetic finger printing is called so because, 1. the blotting is done from the south side 2. Biology MCQ Questions. Multiple Choice Questions on … Ans. This is the biochemistry questions and answers section on "Polymerase Chain Reaction" with explanation for various interview, competitive examination and entrance test. Multiple Choice Questions (MCQ) on Polymerase Chain Reaction quiz answers PDF to prep MCAT practice test. AP PCR fingerprints of … Pcr mcq pdf. Saved from mcqbiology.com. Explanation: A modification of RT – PCR is rapid amplification of cDNA ends or RACE. 1. Who is the father of genetic engineering ? Check the below NCERT MCQ Questions for Class 12 Biology Chapter 12 Biotechnology and its Applications with Answers Pdf free download. How to measure and improve the most important mobile app analytics you need to meet your app's KPIs. 2. PCR. Polymerase chain reaction (PCR) is an efficient and cost-effective molecular tool to copy or amplify small segments of DNA or RNA. EMSA is an electrophoresis technique to detect protein-DNA or protein RNA interactions. Ads Education Learning. Solved examples with detailed answer description, explanation are given and it would be easy to understand. Question 2. Why is PCR so versatile and important ? Biology MCQs for Class 12 Chapter Wise with Answers PDF Download was Prepared Based on Latest Exam Pattern. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Polymerase Chain Reaction 2. Science. Jan 21, 2013 - Biology Multiple Choice Questions and Answers for Different Competitive Exams. B. Question: MCQ 40 Complementary DNA (cDNA) Is Synthesized In A Special RT-PCR Reaction That Yields The CDNA With A Contiguous(uninterrupted) Open Reading Frame Of Exons. Jan 21, 2013 - Biology Multiple Choice Questions and Answers for Different Competitive Exams .. Questions Q.1 – Q.30 belong to this section and carry a total of 50 marks. This process is designed to obtain full length copies of the mRNAs. 4. 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